Optimized protocol for isolation of high-quality single cells from the female mouse reproductive tract tissues for single-cell RNA sequencing

Optimized protocol for isolation of high-quality single cells from the female mouse reproductive tract tissues for single-cell RNA sequencing

Blog Article

Summary: Single-cell RNA sequencing (scRNA-seq) is Aqualamp parts a powerful tool for enumerating the gene expression dynamics at single-cell resolution.Various organs comprising distinct cellular composition and architecture require unique approaches for highly viable single-cell preparation and reliable sequencing results.Here, we describe an optimized protocol for isolating the female reproductive tract (FRT), dissecting different FRT regions, and preparing high-viability single cells from the uterine endocervix and ectocervix to generate a complete molecular cell atlas by scRNA-seq for studying normal physiology and disease.For complete details on the use and execution of this protocol, Multi-Purpose Cleaner please refer to Chumduri et al.

(2021).